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Braz. j. microbiol ; 43(4): 1414-1418, Oct.-Dec. 2012. tab
Artigo em Inglês | LILACS | ID: lil-665827

RESUMO

The effectiveness of free and nanovesicle-encapsulated nisin to control Listeria monocytogenes in Minas Frescal cheese was investigated. Commercial nisin was encapsulated into liposomes of partially purified soy lecithin. Free (0.1 mg/mL and 0.25 mg/mL) and nanovesicle-encapsulated nisin (0.25 mg/mL) were applied onto the surface of cheese samples, and L. monocytogenes was inoculated before incubation at 6-8°C for 28 days. A bactericidal effect was observed with 0.25 mg/mL free nisin; a bacteriostatic effect was observed for liposome-encapsulated nisin and 0.1 mg/mL free nisin. Free nisin was more efficient than nisin-loaded liposomes in controlling L. monocytogenes. Possible reasons for this behavior, and also the significance of nisin to soft cheeses are discussed. Nisin acted as a suitable barrier within hurdle technology, potentially extending the shelf-life and safety of fresh cheeses.


Assuntos
Produtos Fermentados do Leite , Lipossomos , Lisina/análise , Lisina/isolamento & purificação , Listeria monocytogenes/isolamento & purificação , Eficácia , Microbiologia de Alimentos , Amostras de Alimentos , Métodos , Queijo/análise
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